Regulatory
Part:BBa_K2957043:Design
Designed by: Ethan Levy Group: iGEM19_MIT (2019-10-16)
CMV-2xGal4 (Gal4 repressible promoter)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 92
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 92
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 92
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 92
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used Modular Cloning Golden Gate Type II Assembly, so we had to consider how parts came together. See more on the MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT>.
Source
This part was a part in the Weiss lab database.